小主人家境富裕，拥有一屋的玩具。其中他最爱的是牛仔玩偶胡迪，胡迪因此成为众玩具的“老大”。当小主人出门在外时，一屋的玩具自成世界，过着自己的生活。一天，小主人带回了一个新的玩意：太空战警巴斯光年。巴斯光年长相新奇，功能先进，令小主人爱不释手，威胁到了胡迪的地位。胡迪千方百计要赶走巴斯，一不小心二人一起掉出了房间窗口外，邻居的恶狗在狂吠，邻居的小孩是一个玩具虐待狂，胡迪巴斯能不能化敌为友，消灾解难？
They’re back! The Warner brothers, Yakko and Wakko, and the Warner sister Dot, have a great time wreaking havoc and mayhem in the lives of everyone they meet. After returning to their beloved home, the Warner Bros. water tower, the siblings waste no time in causing chaos and comic confusion as they run loose through the studio, turning the world into their personal playground. Joining Yakko, Wakko and Dot, fan-favorite characters Pinky and the Brain also return to continue their quest for world domination.
